Fig. 4

Chronic treatment with 5-BDBD (P2X4R antagonists) inhibited NTG-induced basal and acute hyperalgesia. Prior to NTG/saline administration, mice were treated with vehicle or 5-BDBD (28 mg/kg, i.p.) every other day for 9 days. a Basal hyperalgesia induced by recurrent NTG injection was completely blocked by 5-BDBD. p < 0.01 for drug, time, and interaction; two-way RM ANOVA and Bonferroni post hoc analysis; n = 8/group. ***p < 0.001 compared to the VEH-VEH group. b Every NTG injection evoked acute hyperalgesia, which was also blocked by 5-BDBD. p < 0.01 for drug, no significant effect of time or interaction. Two-way RM ANOVA, n = 8/group