Fig. 6From: Microglia P2X4 receptor contributes to central sensitization following recurrent nitroglycerin stimulationChange in CGRP immunoreactivity in the TNC. a Representative photos of CGRP-immunoreactive staining in the TNC of the VEH, NTG, and NTG + 5-BDBD groups under × 10 objective lens (the upper row) or and × 20 objective lens (the lower row). The white rectangle frame indicates the representative anatomical site observed under × 20objective lens. b Compared to the VEH group, the OD of area covered by CGRP-immunoreactive fibers was decreased in the NTG group. Pretreatment with 5-BDBD attenuated the decrease of CGRP immunopositivity. The OD of staining fibers was not significantly different at the vehicle group and NTG + 5-BDBD group. One-way ANOVA and Tukey’s multiple comparison test. n = 6/group. **p < 0.01 compared to the VEH, ##p < 0.01 compared to the NTG groupBack to article page