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Fig. 3 | Journal of Neuroinflammation

Fig. 3

From: Targeting of the class II transactivator attenuates inflammation and neurodegeneration in an alpha-synuclein model of Parkinson’s disease

Fig. 3

Silencing CIITA dampens α-syn-mediated myeloid MHCII expression. a MHCII expression (MHCII, Ni-DAB, black) in the SNpc surrounding dopaminergic neurons (TH, DAB brown) 4 weeks post-transduction in AAV2-SYN + LGFP (control), AAV2-SYN + LVA, and AAV2-SYN + LVE mice. Both contralateral and ipsilateral sides are shown as intra-group controls. Red squares demonstrate location of the × 20 image (inset). Red arrows denote TH+ neurons (DAB), black arrows mark MHCII+ cells (Ni-DAB). b Quantification of MHCII induction in AAV2-SYN + LGFP, LVA, or LVE treatment groups, calculated using mean gray value of the ipsilateral and contralateral midbrain in ImageJ, adjusting for background. Ipsilateral and contralateral values were used to determine fold induction of MHCII in the ipsilateral side. Data represent the mean MHCII induction, with each data point representing an individual animal. Three tiled midbrain images were taken per animal, with four to six mice per group. Equal numbers of males and females were used, and data were analyzed by a one-way ANOVA with Dunnet’s multiple comparisons, *p < 0.05, **p < 0.005. c Representative flow cytometry plots of mice injected with AAV2-SYN + LGFP, AAV2-SYN + LVA, and AAV2-SYN + LVE at 4 weeks post-transduction. Gates denote MHCII+ microglia. For the flow cytometry experiment, a total of 24 mice were used. Each group consisted of 4 samples, with 2 ventral midbrains pooled per sample. Equal numbers of males and females were used. d Representative MFI curves and quantification of MHCII+ microglia, gated as shown in c. Gates denoting MHCII+ microglia are shown on MFI curves, and the percent of microglia that are MHCII-positive are graphed. For flow cytometry analysis, the mean ± SEM of samples in each group are plotted. One-way ANOVA with Tukey’s multiple comparisons, ****p < 0.0001

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