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Fig. 4 | Journal of Neuroinflammation

Fig. 4

From: Targeting of the class II transactivator attenuates inflammation and neurodegeneration in an alpha-synuclein model of Parkinson’s disease

Fig. 4

Silencing CIITA attenuates α-syn-mediated T cell and monocyte entry. a Representative flow cytometry plots display gating of lymphoid cells (left, representative percent of displayed population is given on plot) followed by CD4 and CD8+ T cells (from the CD45hi CD11b population, right, mean raw cell number ± SEM is shown on plot) in the ventral midbrains of mice bilaterally injected with AAV2-SYN + LGFP, AAV2-SYN + LVA, or AAV2-SYN + LVE at 4 weeks post-transduction. b Quantification of absolute numbers of CD4 and CD8 T cells from a. c Representative flow cytometry plots showing gating on LY6Chi monocytes (from the CD45hi CD11b+ population, mean raw cell number ± SEM is shown on plot) in the ventral midbrains of mice bilaterally injected with AAV2-SYN + LGFP, AAV2-SYN + LVA, or AAV2-SYN + LVE at 4 weeks post-transduction. d Quantification of the LY6Chi monocytes from c. For ad, a total of 24 mice were used—each group consisted of 4 samples with 2 ventral midbrains pooled per sample. Equal numbers of male and female mice were used in these experiments. The mean +/− SEM of samples in each group are plotted. A one-way ANOVA with Tukey’s post hoc test was used for statistical analysis. *p < 0.05, **p < 0.005, ***p < 0.0005

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