Fig. 2

Tryptase can increase PAR-2 levels. Mouse brain microvascular endothelial cells were pre-treated with FS at 400 μM for 30 min and then stimulated with tryptase at 1 μg/mL for 24 h. a Western blotting analysis of PAR-2 levels in which the levels of PAR-2 were quantified and normalised to their respective GAPDH levels. Each value was then expressed relative to the control, which was set to 1 (n = 3). b Cells were stained with anti-PAR-2 antibody and unregulated PAR2-immunopositive expression (green) on the activated bEnd.3 cells was observed using confocal scanning. The blue staining represents DAPI. Scale bar: 25 μm. **P < 0.01 versus the control group. ^##P < 0.01 versus the tryptase group. The data are presented as the mean ± S.E.M of at three separate experiments