Fig. 6

Tryptase activated MAPK and NF-kappa B pathway. FS (400 μM) incubated mouse brain microvascular endothelial cell for 30 min, then tryptase(1 μg/ml) treated for another 30 min. a Western blotting analysis of signalling pathway-associated protein expression. Tryptase activated the ERK and NF-kappa B p65, but FS suppressed the phenomenon. b Expression levels of ERK and NF-kappa B p65 were measured by western blot. c Western blotting analysis of TLR4 levels, which were quantified and normalised to their respective GAPDH levels. d RT-PCR analysis of the relative expression of TNF-α mRNA. e Concentration of TNF-α measured by ELISA. Each value was then expressed relative to the control, which was set to 1 (n = 3). ***P < 0.001, **P < 0.01 versus the control group. ^##P < 0.01, ^#P < 0.05 versus the tryptase group. The data are presented as the mean ± S.E.M of at three separate experiments