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Table 1 Antigenic profile of the human microglial clone 3 cell line

From: The human microglial HMC3 cell line: where do we stand? A systematic literature review

Markers Original characterization CHME3 cells HMC3 cells References
Resting Stimulated Resting Stimulated Resting Stimulated
Myeloid Markers
 CD68 Ki-M7 +++       [17]
 CD68 Ki-M6       [17]
 CD68 EBM/11 ++   +     [17, 35]
 CD68    +++   −/++ IFNγ: ↑ [20, 29, 38]
 CD11b +++   + (and also at mRNA level) 1–42: ↑ IFNγ: ↑ [17, 20, 29, 39, 40]
 CD45      +   [29]
 IBA1      +   [20, 29]
 MHCII IFNγ: ↑ +   −/+ IFNγ: ↑ [17, 20, 29, 37, 39]
 CD14     +   [17, 20, 29]
 MCSF-R    + (western immunoblot)     [41]
 TLR1    +     [40]
 TLR 2 and 6    +++/++ HCV NS3 protein: ↑    [40]
Polarization markers
 CD40    +/++ 1–42: ↓/↑    [37, 39, 43]
 CD80    +     [37]
 CD86    + 1–42: no effect    [37, 39, 43]
 CD163    + 1–42: no effect    [37, 43]
 CD206    + 1–42: no effect    [37, 43]
Chemokine receptors
 CCR1      +   [29]
 CCR2     +   [29, 38]
 CCR3    ++ (MFI) TNFα: ↑
IFNγ: ↑
   [38]
 CCR4      ++   [29]
 CCR5    +   +++   [29, 38]
 CCR10    + (mRNA level) TNFα:↑ (mRNA level)    [38]
 CXCR1    ++ (MFI)   ++   [29, 38]
 CXCR3    +++ (MFI) TNFα: slight ↑
IFNγ: slight↑
+++   [29, 38]
 CX3CR1      +++   [29]
Other markers
 CD4       [17]
 NSE +++       [17]
 GFAP   - (including at mRNA level)    [17, 20, 35, 40]
 NF70KD       [17]
 Α7nAcR    +     [44]
  1. Resting conditions: +, 1–33% of the cell population (or a positive immunocytochemistry); ++, 34–66%; +++, > 66%; −, < 1%. Inflammatory stimuli: ↑, upregulation; ↓, downregulation. MFI, mean fluorescence intensity