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Table 1 Antigenic profile of the human microglial clone 3 cell line

From: The human microglial HMC3 cell line: where do we stand? A systematic literature review

Markers

Original characterization

CHME3 cells

HMC3 cells

References

Resting

Stimulated

Resting

Stimulated

Resting

Stimulated

Myeloid Markers

 CD68 Ki-M7

+++

     

[17]

 CD68 Ki-M6

     

[17]

 CD68 EBM/11

++

 

+

   

[17, 35]

 CD68

  

+++

 

−/++

IFNγ: ↑

[20, 29, 38]

 CD11b

+++

 

+ (and also at mRNA level)

1–42: ↑

IFNγ: ↑

[17, 20, 29, 39, 40]

 CD45

    

+

 

[29]

 IBA1

    

+

 

[20, 29]

 MHCII

IFNγ: ↑

+

 

−/+

IFNγ: ↑

[17, 20, 29, 37, 39]

 CD14

   

+

 

[17, 20, 29]

 MCSF-R

  

+ (western immunoblot)

   

[41]

 TLR1

  

+

   

[40]

 TLR 2 and 6

  

+++/++

HCV NS3 protein: ↑

  

[40]

Polarization markers

 CD40

  

+/++

1–42: ↓/↑

  

[37, 39, 43]

 CD80

  

+

   

[37]

 CD86

  

+

1–42: no effect

  

[37, 39, 43]

 CD163

  

+

1–42: no effect

  

[37, 43]

 CD206

  

+

1–42: no effect

  

[37, 43]

Chemokine receptors

 CCR1

    

+

 

[29]

 CCR2

  

 

+

 

[29, 38]

 CCR3

  

++ (MFI)

TNFα: ↑

IFNγ: ↑

  

[38]

 CCR4

    

++

 

[29]

 CCR5

  

+

 

+++

 

[29, 38]

 CCR10

  

+ (mRNA level)

TNFα:↑ (mRNA level)

  

[38]

 CXCR1

  

++ (MFI)

 

++

 

[29, 38]

 CXCR3

  

+++ (MFI)

TNFα: slight ↑

IFNγ: slight↑

+++

 

[29, 38]

 CX3CR1

    

+++

 

[29]

Other markers

 CD4

     

[17]

 NSE

+++

     

[17]

 GFAP

 

- (including at mRNA level)

 

 

[17, 20, 35, 40]

 NF70KD

     

[17]

 Α7nAcR

  

+

   

[44]

  1. Resting conditions: +, 1–33% of the cell population (or a positive immunocytochemistry); ++, 34–66%; +++, > 66%; −, < 1%. Inflammatory stimuli: ↑, upregulation; ↓, downregulation. MFI, mean fluorescence intensity