Fig. 2

NOD2 stimulation induced dopaminergic degeneration. Wild-type (WT) mice were placed in a stereotaxic device under 1.5% pentobarbital sodium anesthesia and given muramyl dipeptide (MDP, an extrinsic ligand of NOD2) or saline alone into two different sites of the STR separately. a Western blot showed the time-dependent reduction of tyrosine hydroxylase (TH) in WT mouse SN and STR after striatal injection of MDP. b The brain sections from the SN and STR showing TH immunoreactivity on 14 days after striatal infusion of MDP. c The levels of dopamine and its metabolites in the striatum were measured by HPLC/MS analysis on 14 days after MDP injection. All data are presented as the mean ± SEM, n = 6 mice per group. *p < 0.05, compared with indicated group