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Fig. 6 | Journal of Neuroinflammation

Fig. 6

From: NOD2 promotes dopaminergic degeneration regulated by NADPH oxidase 2 in 6-hydroxydopamine model of Parkinson’s disease

Fig. 6

Effect of NOD2 signaling on inflammation in Parkinson’s disease mice induced by 6-OHDA. Wild-type (WT) and NOD2-deficient (NOD2−/−) mice were placed in a stereotaxic device under 1.5% pentobarbital sodium anesthesia and given 6-OHDA, muramyl dipeptide (MDP, an extrinsic ligand of NOD2), or saline alone into two different sites of the STR separately. Western blot analysis was used to measure NF-κB p-p65 (a) and IκBα (b) protein levels in the SN from WT and NOD2−/− mice on 14 days after 6-OHDA or MDP treatment. The chemokine MCP-1 (c) and pro-inflammatory cytokine [IL-6 (d), TNF-α (e), IL-12p70 (f)] and anti-inflammatory cytokine IL-10 (g) on 14 days after striatal infusion of 6-OHDA or MDP in WT and NOD2−/− mice were measured by cytometric bead array (CBA) multiplexed assay. All data are presented as the mean ± SEM, n = 6 mice per group.*p < 0.05 vs. indicated group

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