Fig. 8

NADPH oxidase 2 (NOX2)-derived reactive oxygen species (ROS) was associated with NOD2 signaling in microglia induced by 6-OHDA. Reactive oxygen species (ROS) production (a) and NOD2, NOX2, COX2 and iNOS expressions (b) were induced by 50 μM 6-OHDA time-dependently in microglial BV2 cells. c The NOX2 silence inhibited the expression of NOD2, COX2, and iNOS in microglia induced by 6-OHDA. d Gene silencing of NOX2 in microglia attenuated the toxicity of conditioned medium from 6-OHDA or MDP-stimulated microglia to SH-SY5Y cells. SH-SY5Y cells were treated with conditioned medium from unstimulated microglia (control), 6-OHDA, or MDP-stimulated microglia for 24 h. Cell viability was assessed by the CCK-8 assay. Data are means ± SEM from three independent experiments. *p < 0.05, compared with indicated group