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Fig. 1 | Journal of Neuroinflammation

Fig. 1

From: Complement activation contributes to perioperative neurocognitive disorders in mice

Fig. 1

Study design. a Mice were randomly assigned to three groups: naive, sham, and surgery. Mice were sacrificed for tissues harvesting at 6 h, 1 day, and 3 days after surgery or sham. b Mice were randomly assigned to three groups: sham + vehicle, surgery + vehicle, and surgery + C3aR antagonist (C3aRa). C3aRa or vehicle was given 1 h prior to surgery or sham. Mice were sacrificed for tissues harvesting at 6 h and 1 day after surgery or sham. c Mice were randomly assigned to three groups: sham + vehicle, surgery + vehicle, and surgery + C3aR antagonist (C3aRa). 30 min after C3aRa or vehicle administration, mice were subjected to the training session for trace fear conditioning. Mice underwent surgery or sham 30 min after training. At 3 days after surgery or sham, context test was performed. d Mice were randomly assigned to three groups: sham + vehicle, surgery + vehicle, and surgery + recombinant mouse C3a (rmC3a). Mice were given intranasal rmC3a or vehicle at 24 h and 48 h after surgery or sham procedure. Mice were sacrificed for tissues harvesting at 3 days. e Naïve mice were randomly assigned to vehicle or rmC3a treatment; at 6 h after intranasal rmC3a or vehicle administration, mice were sacrificed for tissue harvesting. f Mice were randomly assigned to three groups: sham + vehicle, surgery + vehicle, and surgery + rmC3a. 30 min after rmC3a or vehicle administration, mice were trained for trace fear conditioning. Mice underwent surgery or sham 30 min after training. The context test was performed at 1 day after surgery or sham procedure

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