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Fig. 2 | Journal of Neuroinflammation

Fig. 2

From: Complement activation contributes to perioperative neurocognitive disorders in mice

Fig. 2

Orthopedic surgery induces complement activation in the hippocampus. a C3 was assayed by ELISA in hippocampal homogenates of naïve and surgery mice (one-way analysis of variance followed by Student-Newman-Keuls test; n = 5). b Representative confocal images of C3 (green) and astrocytic marker GFAP (red) immunostaining in the hippocampus of naïve, surgery, and sham mice on postoperative day 1. c Quantification of C3 occupancy in GFAP+ astrocytes (one-way analysis of variance followed by Tukey post hoc test; n = 5). d Representative confocal images of C3 (green) and microglial marker IBA1 (red) double immunostaining in the hippocampus 1 day after surgery. e Representative confocal images of C3aR (green) and IBA1 (red) labeling in the hippocampus of naïve, surgery, and sham mice at 1 day. f Quantification of C3aR occupancy in IBA1+ microglia, normalized to the level in the naïve group (one-way analysis of variance followed by Tukey post hoc test; n = 5). Scale bar = 30 μm (b, d, and e). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001

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