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Fig. 1 | Journal of Neuroinflammation

Fig. 1

From: Macrophage migration inhibitory factor facilitates production of CCL5 in astrocytes following rat spinal cord injury

Fig. 1

Determination of MIF and CCL5 expression following spinal cord injury. a Western blot analysis of MIF expression following spinal cord contusion at 0d, 1d, 4d, and 7d, respectively. b Quantification data as shown in a; quantities were normalized to endogenous β-actin. c CCL5 protein levels were assayed by ELISA for the injured cord at different time points with or without injection of 8 μl 4-IPP (100 mM) at the lesion sites. d Illustration of section sites showing immunostaining. e Immunostaining of CCL5 in the cross sections of rat contused spinal cord showed colocalization with GFAP-positive cells at 0d and 4d, with or without injection of 4-IPP, respectively. The rectangle indicates region magnified. Error bars represent the standard deviation (P < 0.05). Scale bars, 100 μm

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