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Fig. 2 | Journal of Neuroinflammation

Fig. 2

From: Macrophage migration inhibitory factor facilitates production of CCL5 in astrocytes following rat spinal cord injury

Fig. 2

Binding assay of MIF with CD74 receptor in the primary astrocytes. a Purified primary astrocytes stained with GFAP and Hoechst 33342. b Statistical analysis of primary astrocyte purity. c Immunoprecipitation using anti-His or anti-CD74 antibody and detection of the components of the rMIF- or CD74-associated complexes with anti-CD74 or anti-His antibody. M marker, A/G protein A/G magnetic beads. Scale bars, 50 μm

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