Fig. 6

Determination of intracellular signaling associated with regulation of CCL5 expression. a Interference efficiency of three siRNA oligonucleotides for CD74 was measured by RT-PCR, and siRNA2 was used for the knockdown experiments. b Western blot analysis of pERK, p-P38, pJNK, and p65NFκB following astrocytes treated with siRNA oligonucleotides or scramble for 48 h and then with 2 μg/ml recombinant MIF for 15 min, 30 min, and 60 min, respectively. c Quantification data as shown in a. d Cell supernatants and lysates were tested by ELISA for the production of CCL5 following astrocyte treatment with inhibitor of ERK (PD98059), P38 (SB203580), or JNK (SP600125). Experiments were performed in triplicates. Error bars represent the standard deviation (P < 0.05)