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Fig. 7 | Journal of Neuroinflammation

Fig. 7

From: Macrophage migration inhibitory factor facilitates production of CCL5 in astrocytes following rat spinal cord injury

Fig. 7

Effects of CCL5 on macrophage migration in vitro. a Transwell assay using cell co-culture model. b Interference efficiency of three siRNA oligonucleotides for CCL5 was measured by RT-PCR, and siRNA1 was used for the knockdown experiments. c Migration assay of macrophages co-cultured with astrocytes with or without knockdown of CCL5. Astrocytes were stimulated with 0.5 μg/ml recombinant MIF following CCL5 siRNA interference for 24 h prior to macrophage migration for 48 h. For a transition of macrophages from M1- to M2-phenotype, the cells were incubated in 20 ng/ml recombinant rat IL-13 protein for 2 days. d Quantification data as shown in c. e The expression of M1 and M2 macrophage markers, iNOS and Arginase1, was determined by RT-PCR. Quantities were normalized to endogenous β-actin. Error bars represent the standard deviation. *P < 0.05, #P < 0.05. Scale bars, 100 μm

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