Fig. 12

E2 treatment suppresses LPS-induced Gro1. a Western blot analysis of pituitary and b hippocampus of male mice treated with E2 or placebo pellets for 6 weeks and challenged with LPS or normal saline (NS) as in Fig. 11. Representative blots are shown. c Intensities of protein bands were quantified from three to four individual mice/group, normalized to GAPDH, and presented as percent of control (Placebo/NS). d Gro1 mRNA and IL-1β mRNA levels in the hippocampus. Data are shown as mean ± SEM. Samples from three to four mice/group were run in triplicate and normalized against GAPDH. Results are expressed in fold change vs control (Placebo+NS) taken as 1. *p < 0.05, **p < 0.01 vs control in post-hoc pair-wise t test