Fig. 2

Proinflammatory cytokines induce Gro1 expression in human and murine hippocampal NPC. a mRNA levels of growth factors in hNPC treated with IL-1β (10 ng/mL) for 10 days. Data are shown as mean ± SEM of three independent experiments. All samples from three experiments were run together in triplicate and normalized against GAPDH. Results are expressed in fold change vs untreated control taken as 1; **p < 0.01. b Western blot analysis of Gro1 and NFκB subunits in hNPC treated with IL 1β (10 ng/mL) or IL 6 (50 ng/mL) for 10 days. C, untreated control. Three independent experiments were performed, and representative blots are shown. c Intensities of protein bands were quantified from three individual experiments, normalized to GAPDH, and presented as percent of control (untreated cells); *p < 0.05, **p < 0.01 vs control in post-hoc pair-wise t test. d Western blot analysis of Gro1 in mNPC treated with IL-1β (10 ng/mL) or IL-6 (50 ng/mL) for 5 days. C, untreated control. Three independent experiments were performed, and representative blots are shown. e Intensities of protein bands were quantified from three individual experiments, normalized to GAPDH, and presented as percent of control (untreated cells); *p < 0.05, **p < 0.01 vs control in post-hoc pair-wise t test