Fig. 4

Gro1 induces senescence in neuroblasts. a Western blot analysis of markers of neurogenesis, senescence, apoptosis, and proliferation in murine NPC treated with mGro1 protein (80 ng/mL). Three independent experiments were performed, and representative blots are shown. C, untreated control. b Intensities of protein bands were quantified from three individual experiments, normalized to GAPDH, and presented as percent of control (untreated cells). c DCX mRNA levels in mNPC treated with Gro1. Data are shown as mean ± SEM of three independent experiments. All samples from three experiments were run together in triplicate and normalized against GAPDH. Results are expressed in fold change vs control (untreated cells) taken as 1. *p < 0.05, **p < 0.01 vs control in post-hoc pair-wise t test