Fig. 7

Gro1 induces senescence and arrests proliferation of murine hippocampal neurons. a Western blot analysis of markers of neurogenesis, senescence, apoptosis, and proliferation in HT-22 cells treated with mGro1. Three independent experiments were performed, and representative blots are shown. b Intensities of protein bands were quantified from three individual experiments, normalized to GAPDH, and presented as percent of control (untreated cells). c SA-β-gal enzymatic activity (blue) in HT-22 cells treated with 80 ng/mL Gro1. d Percent SA-β-gal positivity in HT-22 cells treated with Gro1 assessed in 6-well plates in triplicate, with 1000 cells/field counted in three fields/well. Control cells were untreated. *p < 0.05, **p < 0.01 vs control in post-hoc pair-wise t test