Fig. 2

Infant SNI resulted in adolescent anxiety- and depression-like behaviors. Infant rats that received SNI on P10 showed decreased (a) center distance and (b) center duration compared to the sham group from POD 20 to POD 40. c The total distance traveled in the open field did not differ from the sham group. d Infant SNI surgery decreased sucrose intake percentage compared to the sham group on POD 45. In the elevated plus maze test, infant SNI surgery decreased (f) open arm duration compared to the sham group from POD 21 to POD 41 and (e) open arm entries on POD 41. In addition, infant SNI resulted in decreased (g) paw withdrawal threshold from POD 20 to POD 40, increased (h) guarding score from POD 20 to POD 70, and increased (i) cold sensitivity to acetone from POD 20 to POD 40. j SNI decreased the center distance/total distance calculated from the open field test on POD 40. Representative open field and elevated plus maze photos (k). n = 8. “a” denotes the statistically significant difference compared to the sham group