Fig. 7
Increased β-arrestin2 expression restores the anti-inflammatory effects of Drd2 in α-Syn-induced inflammation. The astrocytes from A53T transgenic (A53Ttg/tg) mice were transfected with pcDNA3.1-HA-β-arrestin2 (β-Arr2) or the pcDNA3.1 empty vector (vector) and then treated with quinpirole. a The expression of β-arrestin2 in astrocytes transfected with β-Arr2 for 48 h. Data are presented as the mean ± S.E.M from four independent experiments, t test, **p < 0.01 vs. vector group. The levels of IL-1β were determined by real-time PCR analysis (b) and Elisa analysis (c). Representative immunoblot (d) and quantitative analysis of TLR4 (e), p-IKK (f), and nuclear p65 (g) in astrocytes. Data are presented as the mean ± S.E.M from four independent experiments, two-way ANOVA, *p < 0.05, **p < 0.01 vs. control group, #p < 0.05, ##p < 0.01 vs. quinpirole + vector group