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Fig. 8 | Journal of Neuroinflammation

Fig. 8

From: α-Synuclein disrupts the anti-inflammatory role of Drd2 via interfering β-arrestin2-TAB1 interaction in astrocytes

Fig. 8

α-Synuclein disrupts the β-arrestin2-TAB1 interaction in astrocytes. a Quinpirole enhanced the TAB1-β-arrestin2 interaction and inhibited the TAB1-TAK1 interaction in astrocytes. The interaction of TAB1 with β-arrestin2 and TAK1 in astrocytes treated with quinpirole for 1 h prior to addition of LPS measured by co-IP. b Quinpirole inhibited the LPS-induced TAK1 activation (p-TAK1) evaluated by Western blot analysis. Data are presented as the mean ± S.E.M from four independent experiments, one-way ANOVA, *p < 0.05 vs. control group, and #p < 0.05 vs. LPS treatment group. c α-Syn reduced the TAB1-β-arrestin2 interaction and enhanced the TAB1-TAK1 interaction in astrocytes. The interaction of TAB1 with β-arrestin2 and TAK1 in astrocytes treated with quinpirole for 1 h prior to addition of wide-type (WT) α-Syn or A53T mutant α-Syn measured by co-IP. de Quinpirole failed to inhibit the α-Syn-induced TAK1 activation (p-TAK1) evaluated by Western blot analysis. Representative immunoblot and quantitative analysis of p-TAK1 in astrocytes treated with WT α-Syn (d) or in A53T transgenic (A53Ttg/tg) mice astrocytes (e). Data are presented as the mean ± S.E.M from four independent experiments, two-way ANOVA, *p < 0.05 vs. control group. f A model depicting the roles of α-Syn in disrupting the D2R/β-arrestin2 anti-inflammatory pathway via disassembling of TAB1-β-arrestin2 complex

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