Fig. 4

Effect of PEA-OXA on astrocytes and microglia activation after sciatic nerve crush. The immunofluorescent staining on longitudinal sections of the sciatic nerve for GFAP and Iba-1, showed a significant increase in GFAP and Iba-1 expression in the vehicle group (b, f, respectively) compared to sham mice (a, e, respectively). PEA-OXA treatment significantly reduces the expression for GFAP and Iba-1 compared to the vehicle group, c, g, respectively. The western blot analysis of the lumbar portion of the spinal cord showed the expression and relative densitometric analysis for GFAP and Iba-1 (d, d and h, h1, respectively). Each data are expressed as mean ± SEM from N  =  10 mice for each group. ***p < 0.001 vs corresponding sham. ###p < 0.001 vs. vehicle group