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Fig. 2 | Journal of Neuroinflammation

Fig. 2

From: Microglia prevent peripheral immune cell invasion and promote an anti-inflammatory environment in the brain of APP-PS1 transgenic mice

Fig. 2

Immunohistochemical and flow cytometric analysis of microglia/macrophage cells in the brains of WT and APP-PS1 animals treated with PLX5622. Staining for Iba1 (white) in the hippocampus (a) and the cortex (b) revealed significantly reduced numbers of Iba1+ cells in brains of WT and APP-PS1 mice treated with PLX5622. Surprisingly, higher numbers of Iba1+ cells were observed in both brain regions of APP-PS1 animals before treatment and higher numbers of Iba1+ cells remained resistant to PLX5622 application compared to WT animals (c, d). After 28 days of treatment, microglia were mechanically isolated from total brain hemispheres, stained for CD11b, and quantitatively analyzed via flow cytometry. Representative flow cytometric dot plots of single-living CD11b+ cells isolated from total brain hemispheres (e). Both WT and APP-PS1 animals treated with PLX5622 had highly reduced numbers of CD11b+ cells, and surprisingly APP-PS1 animals had significantly higher numbers of isolated CD11b+ cells compared to WT animals (f). Dapi (blue) was used as nucleus stain. One-way ANOVA with Tukey’s multiple comparison test (c, d n = 6/group and f n = 8–9/group) and unpaired Student’s t test with Welch’s correction was performed comparing only WT with WT + PLX5622 (f, n = 8–9/group). Scale: 100 μm (a, b)

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