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Fig. 4 | Journal of Neuroinflammation

Fig. 4

From: Prostaglandin D2/J2 signaling pathway in a rat model of neuroinflammation displaying progressive parkinsonian-like pathology: potential novel therapeutic targets

Fig. 4

Successive PGJ2 microinfusions induce microglia morphological and functional changes detected post-mortem. a TH (blue, dopaminergic) and Iba1 (red, microglia) immunostaining shows a gradual loss of dopaminergic neurons and gain of activated microglia in the ipsilateral SNpc. Scale bar = 50 μm. b The overall numbers of microglia in the ipsilateral SNpc did not significantly differ between DMSO (control) and PGJ2–treated rats. c Microglia morphologic changes represented by the form factor (FF) calculated as 4π X area/perimeter2. d The number of reactive and amoeboid microglia significantly increased in the ipsilateral SNpc of rats receiving two (2X) PGJ2 injections at 4 weeks but not at 8 weeks post-injections, compared to controls (DMSO-treated). Values on the y-axis represent the ratios between the ipsilateral SNpc over the contralateral, normalized to the number of ramified microglia. Black circles, control, DMSO-treated rats; red circles, PGJ2-treated rats. Statistical significance was estimated with the Student’s T test to compare DMSO and PGJ2-treated groups, and between two PGJ2-treated groups. The p values in red indicate significant (p < 0.05) difference from DMSO-injected rats. N = 3 rats per group. X = number of injections (once per week)

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