Fig. 1

a Experimental design to examine the role of inflammagens on tau seeding activity. Microglia or media alone was plated at DIV0. At DIV5, half of the media was replaced with fresh media containing rTg4510 lysate containing misfolded tau seeds (τ) inflammagen of varying types (i), or media only (Ø). Twenty-four hours later, media was collected for later application to the seeding assay. b Gene expression of various cell type-specific transcripts was quantified by qPCR. Isolated microglia are enriched for the microglia marker CX3CR1 but not markers for astrocytes (Aldh1l1), neurons (Map2, MAPT), oligodendrocytes (MOG), or endothelial cells (PECAM1). Importantly, isolated microglia do not express the gene for tau, MAPT. c Representative fluorescent photomicrograph of Iba1 (red) and DAPI nuclear counterstain (blue) showing typical density and purity of mouse microglia cultures at × 20