Fig. 3
From: The role of microglia in processing and spreading of bioactive tau seeds in Alzheimer’s disease
Tau seeds in microglia protein lysate were quantified using a FRET-based biosensor cell line that detects tau aggregates. a Experimental design. b–d Representative photomicrographs of biosensor cell line 36 h after application of rTg21221, rTg4510, and WT microglia lysate. Bright puncta visible in the rTg4510 sample in (c) are indicative of tau aggregates. e–g Representative flow cytometry plots for measurement of FRET seeding in (e) rTg21221, (f) rTg4510, and (g) WT microglia. h Quantification of FRET seeding by normalized (to untreated wells) integrated FRET density (IFD) and analysis by one-way ANOVA revealed that there was a significant main effect of genotype (p = 0.0003); Bonferroni post-test demonstrated that there is significantly more seeding in the rTg4510 microglia compared to rTg21221 or WT microglia (p < 0.01)