Fig. 3
ZIKV infection stimulates ATF6n and XBP1s to translocate into the cell nuclei of neural cells in the mouse brain. Three-week-old AG6 mice were infected with 1 × 105 PFU/mouse (each dose, N = 3) via intraperitoneal injection. a, b The whole brain sections of AG6 mice were stained with DAPI (blue), anti-ZIKV envelope protein antibody (red), and anti-XBP1 (a) or anti-ATF6 (b) antibody (green) and were used for immunohistofluorescence assay at 5 dpi. Images of the whole brain were captured by using a digital slice scanning analysis system (Pannoramic MIDI). A representative sub-area of mesocephalon is shown. Positive cells marked by white squares were magnified nine times and shown on the top-right corner. c Positive rates of nuclear localization were counted. Data represent three area of mesocephalon through random selection and error bars indicate mean ± SD. Statistical analyses were performed using multiple t tests (N = 3) (P < 0.05 or P < 0.01)