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Fig. 4 | Journal of Neuroinflammation

Fig. 4

From: ZIKV infection activates the IRE1-XBP1 and ATF6 pathways of unfolded protein response in neural cells

Fig. 4

ZIKV infection increases the expression of ER stress sensors in human neural cells. a, b CCF-STTG1and SK-N-SH were infected with ZIKV at an MOI of 5 PFU/cell, and cellular lysates were obtained at 24 and 48 hpi. An equal amount of cell lysates were analyzed with anti-BIP antibody, anti-phospho-eIF2α antibody, anti-total-eIF2α, anti-phoshpo-IRE1 antibody, anti-ATF6n antibody, anti-ZIKV envelope protein antibody, and anti-β-actin antibody. The bands were analyzed by using Image Lab 4.0.1. The fold change of target protein/β-actin was calculated. The protein level between mock-infected and ZIKV-infected compared with internal control β-actin. Data represented three independent experiments and error bars indicate mean ± SD. Statistical analyses were performed using multiple t tests (N = 3) (P < 0.05 or P < 0.01)

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