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Fig. 1 | Journal of Neuroinflammation

Fig. 1

From: α1-antitrypsin mitigates NLRP3-inflammasome activation in amyloid β1–42-stimulated murine astrocytes

Fig. 1

Experimental setting. a Cortices from BALB/c mice were prepared and seeded on flasks 2 weeks prior to stimulation. After a week, when cell confluence was about 80%, flasks were shaken to remove microglia and oligodendrocytes from astrocytes. At passage 2, astrocytes were seeded on experimental plates 48 h prior to stimulation. After short-term stimulation (3 h/ 6 h) with two concentrations of Aβ1–42 (4, 10 μM) or LPS (1 μg/mL) and treatment with A1AT (4 mg/mL) immunocytochemistry, viability assays, caspase 1 assay, RNA and protein isolation were performed. For our control experiments, pretreatment with MCC950 (1 μM) was performed 1 h prior to stimulation. b Astrocyte purity was assessed by ICC using GFAP and Iba1 combined with Hoechst for DNA staining

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