Fig. 2
From: α1-antitrypsin mitigates NLRP3-inflammasome activation in amyloid β1–42-stimulated murine astrocytes
Effect of Aβ1–42 stimulation and A1AT treatment ± MCC950 on cell viability at 3 h and 6 h. Treatment with Aβ1–42 [4 μM], A1AT [4 mg/mL], and MCC950 [1 μM] did not affect LDH-release at 3 h and 6 h stimulation, whereas LPS-stimulation [1 μg/mL] significantly increased LDH-release (a, c). Cell lysis defined maximal (100%; 1.0) release of LDH. MTT-assay (b, d) revealed a trend towards decrease in cell metabolism after 3 h and 6 h Aβ1–42-stimulation, which was not significant. Treatment with MCC950 led to an increase of cell metabolism in each treatment group at 3 h and 6 h, which was not significant. */ap < 0.05; **/aap < 0.01; ***/aaap < 0.001, ns not significant compared to untreated cell control (ctr)