Fig. 5From: α1-antitrypsin mitigates NLRP3-inflammasome activation in amyloid β1–42-stimulated murine astrocytesASC was not regulated by Aβ1–42 and A1AT at 3 h and 6 h stimulation. (a) Quantitative RT-PCR demonstrated ASC was not regulated by Aβ1–42 or A1AT. Data of n = 6 in triplicate represent mean ± SD. (b–c) Western blot revealed no regulation of ASC by Aβ1–42 or A1AT. Data of n = 3 in triplicate represent mean ± SD. (e–f) RT-PCR demonstrated that also at 6 h stimulation time such as MCC950-pretreatment did not regulate ASC mRNA expression. Data of n = 3 in triplicate represent mean ± SD. */ap < 0.05; **/aap < 0.01; ***/aaap < 0.001, ns not significant compared to untreated cell control (ctr)Back to article page