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Fig. 8 | Journal of Neuroinflammation

Fig. 8

From: α1-antitrypsin mitigates NLRP3-inflammasome activation in amyloid β1–42-stimulated murine astrocytes

Fig. 8

A1AT mitigated Aβ1–42-induced upregulation of IL-1β mRNA in primary astrocytes. (ab) As quantified by RT-PCR stimulation with 4 μM Aβ1–42 significantly increased mRNA levels of IL-1β in astrocytes at 3 h and 6 h. Co-treatment with A1AT blocked this increase significantly at 3 h and 6 h. Addition of MCC950 had no significant impact on gene expression at 3 h and 6 h. (c) Aβ1–42-stimulation such as A1AT-treatment did not affect IL-18 mRNA expression at 3 h. (d) Stimulation with Aβ1–42 significantly increased mRNA levels of IL-18 in astrocytes at 6 h. Co-treatment with A1AT did not block this increase. MCC950 had no effects on gene expression of IL-18 at 3 h and 6 h. Data of n = 3 in triplicate represent mean ± SD. */ap < 0.05; **/aap < 0.01; ***/aaap < 0.001, ns not significant compared to untreated cell control (ctr)

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