Table 3 Antibodies for western blotting
From: α1-antitrypsin mitigates NLRP3-inflammasome activation in amyloid β1–42-stimulated murine astrocytes
Antibody | Company | Order no. | Host | Dilution |
|---|---|---|---|---|
Anti-mouse | Sigma-Aldrich, Germany | A4416 | Goat | 1:4000 |
Anti-rabbit | Bio-Rad, USA | 170–6515 | Goat | 1:5000 |
ASC (N15)-R | Santa Cruz, USA | sc-22514-R | Rabbit | 1:1000 |
Caspase 1 | Adipogen | 20B0042C100 | Mouse | 1:1000 |
Caspase 1 p10 | Santa Cruz, USA | sc-514 | Rabbit | 1:1000 |
Caspase 1 p20 | Bioss | bs-6368R | Rabbit | 1:500 |
IL-1β | Abcam, UK | ab9722 | Rabbit | 1:1000 |
IL-1β | Cell Signaling Technology, USA | 12242S | Mouse | 1:1000 |
IL-18 | Abcam, UK | Ab71495 | Rabbit | 1:1000 |
IL-18 | Santa Cruz, USA | sc-7954 | Rabbit | 1:1000 |
NLRP3 | Bioss, USA | bs-10021R | Rabbit | 1:1000 |
β-actin | Santa Cruz, USA | sc-47,778 | Mouse | 1:5000 |