Fig. 1

Expression of MOR and CCR2 are not altered in cell cultures derived from CCR5-null mice. a The CCR5 knockout was verified by the presence of a 280-bp band that represents the neomycin resistance gene insertion into the single coding exon of the receptor. A 230-bp band indicates the presence of the CCR5 receptor in wild-type mice. A 100-bp ladder is shown for reference. b, c MOR and CCR2 protein levels were assessed in both wild-type and CCR5-deficient glia and normalized against GAPDH. d Statistical comparison (two-tailed Student’s t test) demonstrated no significant difference between genotypes in levels of either receptor. e, f Immunocytochemistry of wild-type (e) and knockout (f) glia show similar expression of MOR (red) in GFAP+ (green) astrocytes. Astrocyte morphology varies widely in the cultures. MOR appears as a punctate distribution along the cell surfaces as well as in cytoplasmic areas. Scale bar = 20 μM