Fig. 5

Post-treatment with CA140 significantly reduced LPS-induced IL-1β mRNA levels in the presence of a D1R agonist. a Representative images of proinflammatory cytokine mRNA levels in BV2 microglial cells. b–f BV2 microglial cells were pretreated with LPS (1 μg/mL) or PBS for 30 min, followed by treatment with vehicle (1% DMSO) or A77636 (a D1R agonist, 10 nM) for 5.5 h. Total RNA was then isolated, and proinflammatory cytokine levels were measured by RT-PCR (COX-2, IL-1β, IL-6, iNOS, and TNF-alpha: con, n = 4; LPS, n = 4; LPS + CA140, n = 4). g–h BV2 microglial cells were pretreated with LPS (1 μg/mL) or PBS for 30 min, followed by treatment with vehicle (1% DMSO) or A77636 (10 nM) for 30 min and finally CA140 (10 μM) or vehicle (1% DMSO) for 5 h. Total RNA was then isolated, and IL-1β mRNA levels were measured by RT-PCR (con, n = 6; LPS, n = 6; LPS + CA140, n = 6). *p < 0.05, **p < 0.001