Fig. 9

Pretreatment with LPS followed by CA140 treatment significantly reduced IL-1β and COX-2 levels in wild-type mice. a, c Wild-type mice were injected with LPS (10 mg/kg, i.p.), followed 30 min later by injection twice with CA140 (30 mg/kg, i.p.) at an interval of 1 h and a third injection (30 mg/kg, i.p.) at an interval of 30 min. The mice were perfused, fixed, and immunostained with anti-IL-1β antibody in the cortex (a) and hippocampus (c). b, d, e Quantification of data from a (cortex: con, n = 5 mice; LPS, n = 5 mice; LPS + CA140, n = 5 mice) and c (hippocampus CA1 and DG: con, n = 5 mice; LPS, n = 5 mice; LPS + CA140, n = 5 mice). f–h Wild-type mice were injected with LPS (10 mg/kg, i.p.), followed 30 min later by injection twice with CA140 (30 mg/kg, i.p.) at an interval of 1 h and a third injection (30 mg/kg, i.p.) at an interval of 30 min. The mice were perfused, fixed, and immunostained with anti-IL-1β antibody in the cortex (con, n = 5 mice; LPS, n = 5 mice; LPS + CA140, n = 5 mice), hippocampus CA1 (g, con, n = 5 mice; LPS, n = 5 mice; LPS + CA140, n = 5 mice), and dentate gyrus (h, con, n = 5 mice; LPS, n = 5 mice; LPS + CA140, n = 5 mice). ***p < 0.0001