Fig. 1

HIV-1 Tg26 transgenic mice express viral mRNA in neurogenic regions. a Diagram of the truncated HIV-1 NL4-3 proviral genome in HIV-1 Tg26 mice, showing a deletion of 3.1 kb DNA spanning a majority of the Gag and Pol genes, and the predicted presence of seven viral gene transcripts. b RT-qPCR analysis of viral gene transcripts in neurogenic regions of HIV-1 Tg26 mice. Equal amount of RNA from the SVZs, SGZs, olfactory bulbs (OB), and kidneys of HIV-1 Tg26 mice was reverse transcribed, with 2 ng of the generated cDNA used for real-time PCR with primers covering p17 gag, tat₁ (unspliced variant), tat₂ (spliced variant), env (gp120), vpu, and nef. Samples were collected from four HIV-1 Tg26 mice (two males and two females), and reactions were run in triplicate. Values are expressed as mean ± SEM