Fig. 3

Tg26 mice exhibit hippocampal neurogenesis deficits. a Representative confocal image showing quiescent NSCs (qNSCs, orange arrow, GFAP⁺/Sox2⁺/Ki67⁻), active NSCs (aNSCs, green arrow, GFAP⁺/Sox2⁺/Ki67⁺), and NPCs (dashed arrow, GFAP⁻/Sox2⁺) in the SGZ. b Comparative SGZ images from WT and littermated Tg26 mice showing less aNSCs (green arrows) in Tg26 mice. c Stereological quantification revealed significantly increased numbers of qNSCs, decreased numbers of aNSCs, and decreased numbers of NPCs in Tg26 mouse SGZs. d Representative confocal images showing DCX-positive neuroblasts in WT and Tg26 SGZs. e Stereological quantification revealing less neuroblast formation in Tg26 mice. Data in c and e represent the mean ± SEM from five to six mice per genotype (all females). Scale bars in a, b, and d, 50 μm. *p < 0.05 and **p < 0.01 indicate statistical changes compared with the corresponding WT SGZ