Table 1 List of primers used for RT-qPCR and Tg26 mouse genotyping
From: Adult neurogenic deficits in HIV-1 Tg26 transgenic mice
Gene target | Direction | Sequence (5′ to 3′) |
|---|---|---|
Partial Gag (p17) | T760—forward | GGATAGATGTAAAAGACACCA |
T946—reverse | ACCTGGCTGTTGTTTCCTGTGTC | |
Env (gp120) | T876—forward | CCGAAGGAATAGAAGAAGAAG |
T691—reverse | AGAGTAAGTCTCTCAAGCGG | |
Tat2 (spliced variant) | T1002—forward | TGGAAGCATCCAGGAAGTCAGCC |
T1003—reverse | TTCTTCTTCTATTCCTTCGGGCC | |
Tat1 (unspliced variant) | T1002—forward | TGGAAGCATCCAGGAAGTCAGCC |
T1007—reverse | GAGAAGCTTGATGAGTCTGACTG | |
Nef | F3.3—forward | CCGAAGGAATAGAAGAAGAAG |
R3.3—reverse | CTTGTAGCACCATCCAAAGG | |
Vpu | T1002—forward | TGGAAGCATCCAGGAAGTCAGCC |
R1.3—reverse | GTGGTGGTTGCTTCCTTCC | |
PCR for mouse genotyping | T361—Tg26 forward | GATCTGTGGATCTACCACACACA |
T363—Tg26 reverse | GCTGCTTATATGCAGCATCTGAG |