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Fig. 3 | Journal of Neuroinflammation

Fig. 3

From: Sphingosine 1-phosphate receptor subtype 3 (S1P3) contributes to brain injury after transient focal cerebral ischemia via modulating microglial activation and their M1 polarization

Fig. 3

CAY10444 (CAY) administration attenuates tMCAO-induced microglia activation in 3 days post-ischemic brain. Mice were challenged with tMCAO, and CAY (0.5 mg/kg) was administered intraperitoneally immediately after reperfusion. The effect of CAY on microglial activation was determined by Iba1 immunohistochemistry in 3 days post-ischemic brain. a Representative images of Iba1-immunopositive cells in periischemic (P) and ischemic core (C) regions. Scale bars, 200 μm (top panels) and 50 μm (middle and bottom panels). b Quantification of the number of Iba1-immunopositive cells in both regions. c Quantification of morphological changes of Iba1-positive cells in ischemic core regions (ratio of amoeboid to ramified microglia). n = 5 mice per group. **p < 0.01 and ***p < 0.001 versus sham group. #p < 0.05 and ###p < 0.001 versus vehicle-administered tMCAO group (tMCAO+veh)

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Journal of Neuroinflammation

ISSN: 1742-2094