Fig. 8
Suppression of S1P3 activity attenuates proinflammatory cytokine expression in LPS-stimulated mouse primary microglial cells. Mouse primary microglial cells were stimulated with LPS (100 ng/ml) 30 min after CAY (1 μM) treatment. Alternatively, cells were stimulated with LPS 2 days after transfection with S1P3 shRNA (shS1P3) or non-target control shRNA (shNC). a–c The mRNA expression levels of TNF-α, IL-6, and IL-1β were determined 24 h after LPS treatment using qRT-PCR analysis. n = 4 per group. **p < 0.01 versus vehicle-treated cells. #p < 0.05, ##p < 0.01, and ###p < 0.001 versus LPS-stimulated cells. d–g Effects of S1P3 knockdown (d) on mRNA expression levels of TNF-α, IL-6, and IL-1β (e–g) were determined 24 h after LPS treatment in S1P3 shRNA and shNC transfected cells through qRT-PCR. n = 3 per group. **p < 0.01 versus shNC in d. *p < 0.05, **p < 0.01, and ***p < 0.001 versus vehicle-treated cells (e–g). #p < 0.05, ##p < 0.01, and ###p < 0.001 versus LPS-stimulated cells