Fig. 3

Splenic CD11b levels are significantly decreased after MCAO and LIF treatment. a Western blotting was used to measure levels of CD11b, a marker of activated macrophages/microglia, in the spleen 72 h after sham surgery and MCAO. Levels of CD11B were significantly lower in the MCAO + PBS group (**p < 0.01) and the MCAO + LIF (***p < 0.001) were significantly lower than the sham group. b When CD11b levels were normalized to spleen weight, there was a significant decrease in normalized CD11b after LIF treatment compared to sham levels (**p < 0.01). c According to the results of the Pearson correlation analysis, there was a significant correlation between spleen weights and CD11b levels in the spleen (*p < 0.05). n = 8 animals per treatment group