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Fig. 1 | Journal of Neuroinflammation

Fig. 1

From: Reduction of SIRT1 epigenetically upregulates NALP1 expression and contributes to neuropathic pain induced by chemotherapeutic drug bortezomib

Fig. 1

Increased NALP1 in spinal dorsal horn is involved in bortezomib-induced mechanical allodynia. a Intraperitoneal administration of bortezomib (0.4 mg/kg for five consecutive days) markedly decreased the mechanical withdrawal threshold. n = 12 in each group, **P < 0.01 versus vehicle group. The level of NALP1 protein (b) and mRNA (c) in dorsal horn was significantly upregulated following BTZ treatment. n = 6 in each group, **P < 0.01 versus the vehicle group. d The photographs showed the double staining between neuronal markers (NeuN, red) or astrocyte marker (GFAP, red) or microglia marker (OX42, red) with NALP1 (green) in spinal cord of rats on day 10 following BTZ treatment. n = 4 in each group; scale bar, 50 μm. The expression of NALP1 mRNA (e) and protein (f) in dorsal horn were suppressed on day 10 following consecutive intrathecal injection of NALP1 siRNA for 10 days. n = 5 in each group, **P < 0.01 versus corresponding scramble siRNA group. g Intrathecal delivery of NALP1 siRNA for 10 days attenuated the mechanical allodynia following BTZ treatment. n = 12 in each group, **P < 0.01 versus vehicle group, ##P < 0.01 versus corresponding scramble siRNA group

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