Fig. 2

The decrease of SIRT1 in spinal dorsal horn participated to bortezomib-induced mechanical allodynia. a Representative histogram and blots revealed the decrease of SIRT1 in spinal dorsal horn following BTZ treatment. n = 6 in each group, **P < 0.01 versus the vehicle group. b The photographs showed double staining between neuronal markers (NeuN, green) or astrocyte marker (GFAP, green) or microglia marker (OX42, green) with SIRT1 (red) in spinal cord of rats on day 10 following BTZ treatment. n = 3 in each group; scale bar, 100 μm. c Continuous intrathecal injection of resveratrol at dose of 500 μg/10 μl or 250 μg/10 μl, but not 50 μg/10 μl for 10 days ameliorated mechanical allodynia following BTZ treatment. n = 12 in each group, **P < 0.01 versus the vehicle group, ^##P < 0.01 versus corresponding vehicle + bortezomib group. d Intrathecal injection of SIRT1 siRNA downregulated the SIRT1 expression in dorsal horn. n = 3 in each group, **P < 0.01 the control group, ^##P < 0.01 versus corresponding siRNA group. e Downregulation of the SIRT1 by SIRT1 siRNA induced the mechanical allodynia in normal rats. n = 8 in each group, **P < 0.01 the control group