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Table 1 Real-time PCR primers

From: Reduced gut microbiome protects from alcohol-induced neuroinflammation and alters intestinal and brain inflammasome expression

Primer

Forward (5′–3′)

Reverse (5′–3′)

18S

GTAACCCGTTGAACCCCATT

CCATCCAATCGGTAGTAGCG

16S

TCCTACGGGAGGCAGCAGT

GGACTACCAGGGTATCTAATCCTGTT

Tnfα

GAAGTTCCCAAATGGCCTCC

GTGAGGGTCTGGGCCATAGA

Mcp-1

CAG GTC CCT GTC ATG CTT CT

TCTGGACCCATTCCTTCTTG

Il-1β

TCTTTGAAGTTGACGGACCC

TGAGTGATACTGCCTGCCTG

Il-17

CAGGGAGAGCTTCATCTGTGT

GCTGAGCTTTGAGGGATGAT

Il-23

AAGTTCTCTCCTCTTCCCTGTCGC

TCTTGTGGAGCAGCAGATGTGAG

Hmgb1

CGCGGAGGAAAATCAACTAA

TCATAACGAGCCTTGTCAGC

Il-6

ACAACCACGGCCTTCCCTACTT

CACGATTTCCCAGAGAACATGTG

Cox2

AACCGAGTCGTTCTGCCAAT

CTAGGGAGGGGACTGCTCAT

Nlrp3

AGCCTTCCAGGATCCTCTTC

CTTGGGCAGCAGTTTCTTTC

Asc

GAAGCTGCTGACAGTGCAAC

GCCACAGCTCCAGACTCTTC

Casp1

AGATGGCACATTTCCAGGAC

GATCCTCCAGCAGCAACTTC

Il-18

CAGGCCTGACATCTTCTGCAA

TCTGACATGGCAGCCATTGT

  1. The above forward and reverse sequences of primers were used in real-time PCR
  2. Abbreviations: Tnfα tumor necrosis factor-α, Mcp-1 monocyte chemoattractant protein 1 (encoded by CCL2), Il-1β interleukin-1β, Il-17 interleukin-17, Il-23 interleukin-23, Hmgb1 high-mobility group box 1, Il-6 interleukin 6, Cox2 cyclooxygenase 2, Nlrp3 NLR family pyrin domain containing 3, Asc apoptosis-associated speck-like protein (encoded by PYCARD), Casp1 caspase-1 (encoded by CASP1), Il-18 interleukin-18