Fig. 2

High-fat feeding and hyperglycemia contribute to global immune changes in the brain after 9 weeks. a Flow cytometry analysis of brain CD45⁺7AAD⁻ leukocytes of the left cerebral hemisphere. b Gated cells were analysed for the expression of CD11b as a marker of neutrophils, monocytes and microglia. c CD11b⁺ cell gate was further analysed for expression of CD68 as a similar marker. d Gated cells for B220 as a marker of B cells and (e) CD3 as a marker of T cells were analysed from the live CD45⁺ population. Data are shown as mean ± S.E.M. of a–e, n = 9–10 per group. Data in a, b, d, e analysed by one-way ANOVA with Dunnett’s. c Analysed by Kruskal-Wallis with Dunn’s. *P < 0.05, **P < 0.01 vs CIT + CON control group