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Fig. 2 | Journal of Neuroinflammation

Fig. 2

From: The IFN-γ/PD-L1 axis between T cells and tumor microenvironment: hints for glioma anti-PD-1/PD-L1 therapy

Fig. 2

The distribution of tumor-infiltrating T cells and PD-L1 in murine glioma model. a Murine glioma is divided into four parts according to the density and morphological characteristics of Iba1+ myeloid cells. Representative staining for Iba1 (green) and DAPI (blue) in the tumor-bearing brain (day 20). N, normal tissue; TR, tumor rim; IM, invasive margin; IT, intratumoral region. Scale bar, 500 μm. b Representative staining for Iba1 (green), CD4 (red) or CD8 (red), and DAPI (blue) in the tumor-bearing brain (day 20); different regions of the tumor-bearing brain were shown. Scale bar, 100 μm. c CD4+ cell or CD8+ cell counts per field were calculated. Data were collected from five random fields for each region per mouse, n = 4. One-way ANOVA was performed. *p < 0.05; **p < 0.01. All values are shown as mean ± SEM. d Representative staining for PD-L1 (red) and DAPI (blue) in the tumor-bearing brain (day 20). Scale bar, 200 μm. e Representative staining for Iba1 (green) and PD-L1 (red) in the IT area of tumor-bearing brain (day 20). Scale bar, 50 μm

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