Fig. 1

Ifitm3 regulates the extracellular level of Fstl1. a Representative western blot images of Fstl1 protein expression in cell lysates and ACM derived from WT and Ifitm3 KO astrocytes 24 h after polyI:C treatment. b, c Fstl1 protein levels in cell lysates (b) and ACM (c) derived from WT and Ifitm3 KO astrocytes after polyI:C treatment. Values indicate the means ± SE (n = 3). *p < 0.05, ^#p < 0.05, and **p < 0.01 versus the respective control treatment. d The extracellular level of Fstl1 in conditioned medium after Fstl1 and Ifitm3 overexpression. COS7 cells were co-transfected with Fstl1, Ifitm3, and control vectors (mock) as indicated respectively. Overexpression of Ifitm3 increased Fstl1 protein in CM without affecting Fstl1 expression in COS7 cell lysates. Data show the mean ± SE (n = 6). *p < 0.05 versus Fstl1/mock. CM, conditioned medium